Construct: shRNA TRCN0000234443

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019980.1-881s21c1
DNA Barcode:
CACTGTTTGGCTTGATCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Litaf (56722)
Gene Description:
LPS-induced TN factor
Transcript:
RefSeq NM_019980.1 (NON-CURRENT)
Match location:
Position 881 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234443)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTGTTTGGCTTGATCTATT
Hairpin Sequence:
5'-CCGG-CACTGTTTGGCTTGATCTATT-CTCGAG-AATAGATCAAGCCAAACAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTGTTTGGCTTGATCTATTCTCGAGAATAGATCAAGCCAAACAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTGTTTGGCTTGATCTATTCTCGAGAATAGATCAAGCCAAACAGTG-3'

Other clones with same target sequence:

(none)