Construct: shRNA TRCN0000234500

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001002255.1-479s21c1
DNA Barcode:
TACATGAAGTAACTGGTATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SUMO4 (387082)
Gene Description:
small ubiquitin like modifier 4
Transcript:
RefSeq NM_001002255.1 (NON-CURRENT)
Match location:
Position 479 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234500)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACATGAAGTAACTGGTATGT
Hairpin Sequence:
5'-CCGG-TACATGAAGTAACTGGTATGT-CTCGAG-ACATACCAGTTACTTCATGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACATGAAGTAACTGGTATGTCTCGAGACATACCAGTTACTTCATGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACATGAAGTAACTGGTATGTCTCGAGACATACCAGTTACTTCATGTA-3'

Other clones with same target sequence:

(none)