Construct: shRNA TRCN0000234515

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003101.4-2688s21c1
DNA Barcode:
TAATCACCTCCACTCATATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SOAT1 (6646)
Gene Description:
sterol O-acyltransferase 1
Transcript:
RefSeq NM_003101.4 (NON-CURRENT)
Match location:
Position 2688 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234515)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAATCACCTCCACTCATATTA
Hairpin Sequence:
5'-CCGG-TAATCACCTCCACTCATATTA-CTCGAG-TAATATGAGTGGAGGTGATTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAATCACCTCCACTCATATTACTCGAGTAATATGAGTGGAGGTGATTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAATCACCTCCACTCATATTACTCGAGTAATATGAGTGGAGGTGATTA-3'

Other clones with same target sequence:

(none)