Construct: shRNA TRCN0000234534

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001903.2-1471s21c1
DNA Barcode:
CCCTCTGTCCTCAGGTTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CTNNA1 (1495)
Gene Description:
catenin alpha 1
Transcript:
RefSeq NM_001903.2 (NON-CURRENT)
Match location:
Position 1471 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234534)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCTGTCCTCAGGTTATTA
Hairpin Sequence:
5'-CCGG-CCCTCTGTCCTCAGGTTATTA-CTCGAG-TAATAACCTGAGGACAGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCTGTCCTCAGGTTATTACTCGAGTAATAACCTGAGGACAGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCTGTCCTCAGGTTATTACTCGAGTAATAACCTGAGGACAGAGGG-3'

Other clones with same target sequence:

(none)