Construct: shRNA TRCN0000234553

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017780.2-6333s21c1
DNA Barcode:
AGCGGAAAGGGAAGCTATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHD7 (55636)
Gene Description:
chromodomain helicase DNA binding protein 7
Transcript:
RefSeq NM_017780.2 (NON-CURRENT)
Match location:
Position 6333 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234553)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCGGAAAGGGAAGCTATTAT
Hairpin Sequence:
5'-CCGG-AGCGGAAAGGGAAGCTATTAT-CTCGAG-ATAATAGCTTCCCTTTCCGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCGGAAAGGGAAGCTATTATCTCGAGATAATAGCTTCCCTTTCCGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCGGAAAGGGAAGCTATTATCTCGAGATAATAGCTTCCCTTTCCGCT-3'

Other clones with same target sequence:

(none)