Construct: shRNA TRCN0000234567

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003560.2-2830s21c1
DNA Barcode:
CCCATCAGCTCAAACATTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLA2G6 (8398)
Gene Description:
phospholipase A2 group VI
Transcript:
RefSeq NM_003560.2 (NON-CURRENT)
Match location:
Position 2830 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234567)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATCAGCTCAAACATTAAG
Hairpin Sequence:
5'-CCGG-CCCATCAGCTCAAACATTAAG-CTCGAG-CTTAATGTTTGAGCTGATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATCAGCTCAAACATTAAGCTCGAGCTTAATGTTTGAGCTGATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATCAGCTCAAACATTAAGCTCGAGCTTAATGTTTGAGCTGATGGG-3'

Other clones with same target sequence:

(none)