Construct: shRNA TRCN0000234574

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001129.3-1236s21c1
DNA Barcode:
TACGATGACATGGACTATTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AEBP1 (165)
Gene Description:
AE binding protein 1
Transcript:
RefSeq NM_001129.3 (NON-CURRENT)
Match location:
Position 1236 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234574)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACGATGACATGGACTATTAC
Hairpin Sequence:
5'-CCGG-TACGATGACATGGACTATTAC-CTCGAG-GTAATAGTCCATGTCATCGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACGATGACATGGACTATTACCTCGAGGTAATAGTCCATGTCATCGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACGATGACATGGACTATTACCTCGAGGTAATAGTCCATGTCATCGTA-3'

Other clones with same target sequence:

(none)