Construct: shRNA TRCN0000234605

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015260.1-4303s21c1
DNA Barcode:
TGTATACACGCACAGATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIN3B (23309)
Gene Description:
SIN3 transcription regulator family member B
Transcript:
RefSeq NM_015260.1 (NON-CURRENT)
Match location:
Position 4303 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234605)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTATACACGCACAGATATTT
Hairpin Sequence:
5'-CCGG-TGTATACACGCACAGATATTT-CTCGAG-AAATATCTGTGCGTGTATACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTATACACGCACAGATATTTCTCGAGAAATATCTGTGCGTGTATACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTATACACGCACAGATATTTCTCGAGAAATATCTGTGCGTGTATACA-3'

Other clones with same target sequence:

(none)