Construct: shRNA TRCN0000234615

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020245.3-7152s21c1
DNA Barcode:
AGTGTTCACCAGGGTATTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TULP4 (56995)
Gene Description:
TUB like protein 4
Transcript:
RefSeq NM_020245.3 (NON-CURRENT)
Match location:
Position 7152 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234615)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGTTCACCAGGGTATTAAA
Hairpin Sequence:
5'-CCGG-AGTGTTCACCAGGGTATTAAA-CTCGAG-TTTAATACCCTGGTGAACACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGTTCACCAGGGTATTAAACTCGAGTTTAATACCCTGGTGAACACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGTTCACCAGGGTATTAAACTCGAGTTTAATACCCTGGTGAACACT-3'

Other clones with same target sequence:

(none)