Construct: shRNA TRCN0000234634

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002918.3-2357s21c1
DNA Barcode:
ACCCAAGCGATCCGGAACTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RFX1 (5989)
Gene Description:
regulatory factor X1
Transcript:
RefSeq NM_002918.3 (NON-CURRENT)
Match location:
Position 2357 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234634)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCAAGCGATCCGGAACTTT
Hairpin Sequence:
5'-CCGG-ACCCAAGCGATCCGGAACTTT-CTCGAG-AAAGTTCCGGATCGCTTGGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCAAGCGATCCGGAACTTTCTCGAGAAAGTTCCGGATCGCTTGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCAAGCGATCCGGAACTTTCTCGAGAAAGTTCCGGATCGCTTGGGT-3'

Other clones with same target sequence:

(none)