Construct: shRNA TRCN0000234642

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021937.3-564s21c1
DNA Barcode:
TTCCGAGGTGCACCGATTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EEFSEC (60678)
Gene Description:
eukaryotic elongation factor, selenocysteine-tRNA specific
Transcript:
RefSeq NM_021937.3 (NON-CURRENT)
Match location:
Position 564 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234642)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCCGAGGTGCACCGATTATA
Hairpin Sequence:
5'-CCGG-TTCCGAGGTGCACCGATTATA-CTCGAG-TATAATCGGTGCACCTCGGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCCGAGGTGCACCGATTATACTCGAGTATAATCGGTGCACCTCGGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCCGAGGTGCACCGATTATACTCGAGTATAATCGGTGCACCTCGGAA-3'

Other clones with same target sequence:

(none)