Construct: shRNA TRCN0000234652

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005374.3-1073s21c1
DNA Barcode:
GGACTTGCTCCAGATCGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MPP2 (4355)
Gene Description:
membrane palmitoylated protein 2
Transcript:
RefSeq NM_005374.3 (NON-CURRENT)
Match location:
Position 1073 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234652)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGACTTGCTCCAGATCGTAAA
Hairpin Sequence:
5'-CCGG-GGACTTGCTCCAGATCGTAAA-CTCGAG-TTTACGATCTGGAGCAAGTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGACTTGCTCCAGATCGTAAACTCGAGTTTACGATCTGGAGCAAGTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGACTTGCTCCAGATCGTAAACTCGAGTTTACGATCTGGAGCAAGTCC-3'

Other clones with same target sequence:

(none)