Construct: shRNA TRCN0000234693

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032111.2-294s21c1
DNA Barcode:
TCGCTGCATCCATGTCTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRPL14 (64928)
Gene Description:
mitochondrial ribosomal protein L14
Transcript:
RefSeq NM_032111.2 (NON-CURRENT)
Match location:
Position 294 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234693)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGCTGCATCCATGTCTATAA
Hairpin Sequence:
5'-CCGG-TCGCTGCATCCATGTCTATAA-CTCGAG-TTATAGACATGGATGCAGCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGCTGCATCCATGTCTATAACTCGAGTTATAGACATGGATGCAGCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGCTGCATCCATGTCTATAACTCGAGTTATAGACATGGATGCAGCGA-3'

Other clones with same target sequence:

(none)