Construct: shRNA TRCN0000234708

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198486.2-323s21c1
DNA Barcode:
CATTGCAAGACTTCGCCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPL7L1 (285855)
Gene Description:
ribosomal protein L7 like 1
Transcript:
RefSeq NM_198486.2 (NON-CURRENT)
Match location:
Position 323 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234708)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTGCAAGACTTCGCCTAAA
Hairpin Sequence:
5'-CCGG-CATTGCAAGACTTCGCCTAAA-CTCGAG-TTTAGGCGAAGTCTTGCAATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTGCAAGACTTCGCCTAAACTCGAGTTTAGGCGAAGTCTTGCAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTGCAAGACTTCGCCTAAACTCGAGTTTAGGCGAAGTCTTGCAATG-3'

Other clones with same target sequence:

(none)