Construct: shRNA TRCN0000234725

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005966.3-3583s21c1
DNA Barcode:
CAGATCACTGTCACGTATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NAB1 (4664)
Gene Description:
NGFI-A binding protein 1
Transcript:
RefSeq NM_005966.3 (NON-CURRENT)
Match location:
Position 3583 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234725)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATCACTGTCACGTATATA
Hairpin Sequence:
5'-CCGG-CAGATCACTGTCACGTATATA-CTCGAG-TATATACGTGACAGTGATCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATCACTGTCACGTATATACTCGAGTATATACGTGACAGTGATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATCACTGTCACGTATATACTCGAGTATATACGTGACAGTGATCTG-3'

Other clones with same target sequence:

(none)