Construct: shRNA TRCN0000234775

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005223.3-2383s21c1
DNA Barcode:
ACTGGGACGGAACAGCTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DNASE1 (1773)
Gene Description:
deoxyribonuclease 1
Transcript:
RefSeq NM_005223.3 (CURRENT)
Match location:
Position 2383 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234775)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGGGACGGAACAGCTATAA
Hairpin Sequence:
5'-CCGG-ACTGGGACGGAACAGCTATAA-CTCGAG-TTATAGCTGTTCCGTCCCAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGGGACGGAACAGCTATAACTCGAGTTATAGCTGTTCCGTCCCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGGGACGGAACAGCTATAACTCGAGTTATAGCTGTTCCGTCCCAGT-3'

Other clones with same target sequence:

(none)