Construct: shRNA TRCN0000234801

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029045.1-1350s21c1
DNA Barcode:
GCTGGAGATTCCGGCCATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4930432K21Rik (74666)
Gene Description:
RIKEN cDNA 4930432K21 gene
Transcript:
RefSeq NM_029045.1 (NON-CURRENT)
Match location:
Position 1350 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234801)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGAGATTCCGGCCATATA
Hairpin Sequence:
5'-CCGG-GCTGGAGATTCCGGCCATATA-CTCGAG-TATATGGCCGGAATCTCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGAGATTCCGGCCATATACTCGAGTATATGGCCGGAATCTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGAGATTCCGGCCATATACTCGAGTATATGGCCGGAATCTCCAGC-3'

Other clones with same target sequence:

(none)