Construct: shRNA TRCN0000234811

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002779.3-3103s21c1
DNA Barcode:
CCTGGATCACTCGCATCAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSD (5662)
Gene Description:
pleckstrin and Sec7 domain containing
Transcript:
RefSeq NM_002779.3 (NON-CURRENT)
Match location:
Position 3103 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234811)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGATCACTCGCATCAATG
Hairpin Sequence:
5'-CCGG-CCTGGATCACTCGCATCAATG-CTCGAG-CATTGATGCGAGTGATCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGATCACTCGCATCAATGCTCGAGCATTGATGCGAGTGATCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGATCACTCGCATCAATGCTCGAGCATTGATGCGAGTGATCCAGG-3'

Other clones with same target sequence:

(none)