Construct: shRNA TRCN0000234838

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016933.3-629s21c1
DNA Barcode:
CTCCGGGTCACTGCACTATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptprcap (19265)
Gene Description:
protein tyrosine phosphatase, receptor type, C polypeptide-associated protein
Transcript:
RefSeq NM_016933.3 (CURRENT)
Match location:
Position 629 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234838)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCGGGTCACTGCACTATAG
Hairpin Sequence:
5'-CCGG-CTCCGGGTCACTGCACTATAG-CTCGAG-CTATAGTGCAGTGACCCGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCGGGTCACTGCACTATAGCTCGAGCTATAGTGCAGTGACCCGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCGGGTCACTGCACTATAGCTCGAGCTATAGTGCAGTGACCCGGAG-3'

Other clones with same target sequence:

(none)