Construct: shRNA TRCN0000234845

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013302.3-1860s21c1
DNA Barcode:
CATGGCCACTCATACAGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EEF2K (29904)
Gene Description:
eukaryotic elongation factor 2 kinase
Transcript:
RefSeq NM_013302.3 (NON-CURRENT)
Match location:
Position 1860 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234845)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGGCCACTCATACAGTAAT
Hairpin Sequence:
5'-CCGG-CATGGCCACTCATACAGTAAT-CTCGAG-ATTACTGTATGAGTGGCCATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGGCCACTCATACAGTAATCTCGAGATTACTGTATGAGTGGCCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGGCCACTCATACAGTAATCTCGAGATTACTGTATGAGTGGCCATG-3'

Other clones with same target sequence:

(none)