Construct: shRNA TRCN0000234901

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005121.2-3653s21c1
DNA Barcode:
ACAAGATCAGTGCACTAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MED13 (9969)
Gene Description:
mediator complex subunit 13
Transcript:
RefSeq NM_005121.2 (NON-CURRENT)
Match location:
Position 3653 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234901)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAAGATCAGTGCACTAATTT
Hairpin Sequence:
5'-CCGG-ACAAGATCAGTGCACTAATTT-CTCGAG-AAATTAGTGCACTGATCTTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAAGATCAGTGCACTAATTTCTCGAGAAATTAGTGCACTGATCTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAAGATCAGTGCACTAATTTCTCGAGAAATTAGTGCACTGATCTTGT-3'

Other clones with same target sequence:

(none)