Construct: shRNA TRCN0000234952

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039891.1-1961s21c1
DNA Barcode:
TACTTGAGAGTGGGATAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF674 (641339)
Gene Description:
zinc finger protein 674
Transcript:
RefSeq NM_001039891.1 (NON-CURRENT)
Match location:
Position 1961 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234952)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACTTGAGAGTGGGATAATTA
Hairpin Sequence:
5'-CCGG-TACTTGAGAGTGGGATAATTA-CTCGAG-TAATTATCCCACTCTCAAGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACTTGAGAGTGGGATAATTACTCGAGTAATTATCCCACTCTCAAGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACTTGAGAGTGGGATAATTACTCGAGTAATTATCCCACTCTCAAGTA-3'

Other clones with same target sequence:

(none)