Construct: shRNA TRCN0000234967

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033360.2-2867s21c1
DNA Barcode:
TTATCTCTGTACCGTAATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpr101 (245424)
Gene Description:
G protein-coupled receptor 101
Transcript:
RefSeq NM_001033360.2 (NON-CURRENT)
Match location:
Position 2867 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000234967)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTATCTCTGTACCGTAATAAA
Hairpin Sequence:
5'-CCGG-TTATCTCTGTACCGTAATAAA-CTCGAG-TTTATTACGGTACAGAGATAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTATCTCTGTACCGTAATAAACTCGAGTTTATTACGGTACAGAGATAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTATCTCTGTACCGTAATAAACTCGAGTTTATTACGGTACAGAGATAA-3'

Other clones with same target sequence:

(none)