Construct: shRNA TRCN0000235018

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006020.2-555s21c1
DNA Barcode:
GAAATACTCAGCAGATCATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ALKBH1 (8846)
Gene Description:
alkB homolog 1, histone H2A dioxygenase
Transcript:
RefSeq NM_006020.2 (NON-CURRENT)
Match location:
Position 555 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235018)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAATACTCAGCAGATCATTA
Hairpin Sequence:
5'-CCGG-GAAATACTCAGCAGATCATTA-CTCGAG-TAATGATCTGCTGAGTATTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAATACTCAGCAGATCATTACTCGAGTAATGATCTGCTGAGTATTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAATACTCAGCAGATCATTACTCGAGTAATGATCTGCTGAGTATTTC-3'

Other clones with same target sequence:

  1. TRCN0000239212