Construct: shRNA TRCN0000235047

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015061.2-903s21c1
DNA Barcode:
ATACTTGGATTACGAAGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KDM4C (23081)
Gene Description:
lysine demethylase 4C
Transcript:
RefSeq NM_015061.2 (NON-CURRENT)
Match location:
Position 903 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235047)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATACTTGGATTACGAAGATTT
Hairpin Sequence:
5'-CCGG-ATACTTGGATTACGAAGATTT-CTCGAG-AAATCTTCGTAATCCAAGTAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATACTTGGATTACGAAGATTTCTCGAGAAATCTTCGTAATCCAAGTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATACTTGGATTACGAAGATTTCTCGAGAAATCTTCGTAATCCAAGTAT-3'

Other clones with same target sequence:

(none)