Construct: shRNA TRCN0000235074

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025259.4-2529s21c1
DNA Barcode:
AGACATTAGTGGATAAGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MSH5 (4439)
Gene Description:
mutS homolog 5
Transcript:
RefSeq NM_025259.4 (NON-CURRENT)
Match location:
Position 2529 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235074)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACATTAGTGGATAAGTTTA
Hairpin Sequence:
5'-CCGG-AGACATTAGTGGATAAGTTTA-CTCGAG-TAAACTTATCCACTAATGTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACATTAGTGGATAAGTTTACTCGAGTAAACTTATCCACTAATGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACATTAGTGGATAAGTTTACTCGAGTAAACTTATCCACTAATGTCT-3'

Other clones with same target sequence:

(none)