Construct: shRNA TRCN0000235135

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005585.3-1866s21c1
DNA Barcode:
TGAAACGGAGGCTACCAACTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMAD6 (4091)
Gene Description:
SMAD family member 6
Transcript:
RefSeq NM_005585.3 (NON-CURRENT)
Match location:
Position 1866 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235135)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAAACGGAGGCTACCAACTC
Hairpin Sequence:
5'-CCGG-TGAAACGGAGGCTACCAACTC-CTCGAG-GAGTTGGTAGCCTCCGTTTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAAACGGAGGCTACCAACTCCTCGAGGAGTTGGTAGCCTCCGTTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAAACGGAGGCTACCAACTCCTCGAGGAGTTGGTAGCCTCCGTTTCA-3'

Other clones with same target sequence:

(none)