Construct: shRNA TRCN0000235198

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001004283.1-1433s21c1
DNA Barcode:
GAGCAACACTCTGAGTTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm14306 (668009)
Gene Description:
novel KRAB box and zinc finger, C2H2 type domain containing protein
Transcript:
RefSeq XM_001004283.1 (NON-CURRENT)
Match location:
Position 1433 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235198)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCAACACTCTGAGTTTATT
Hairpin Sequence:
5'-CCGG-GAGCAACACTCTGAGTTTATT-CTCGAG-AATAAACTCAGAGTGTTGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCAACACTCTGAGTTTATTCTCGAGAATAAACTCAGAGTGTTGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCAACACTCTGAGTTTATTCTCGAGAATAAACTCAGAGTGTTGCTC-3'

Other clones with same target sequence:

(none)