Construct: shRNA TRCN0000235202
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_001004283.1-2570s21c1
- DNA Barcode:
- GCAGTACTCTCCAGATATATA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Gm14306 (668009)
- Gene Description:
- novel KRAB box and zinc finger, C2H2 type domain containing protein
- Transcript:
- RefSeq XM_001004283.1 (NON-CURRENT)
- Match location:
- Position 2570 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000235202)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAGTACTCTCCAGATATATA
- Hairpin Sequence:
-
5'-CCGG-GCAGTACTCTCCAGATATATA-CTCGAG-TATATATCTGGAGAGTACTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAGTACTCTCCAGATATATACTCGAGTATATATCTGGAGAGTACTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAGTACTCTCCAGATATATACTCGAGTATATATCTGGAGAGTACTGC-3'
Other clones with same target sequence:
(none)
