Construct: shRNA TRCN0000235215

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_974632.1-643s21c1
DNA Barcode:
CTAGACACTGAGGGCCGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
EG665105 (665105)
Gene Description:
predicted gene, EG665105
Transcript:
RefSeq XM_974632.1 (NON-CURRENT)
Match location:
Position 643 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235215)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAGACACTGAGGGCCGATAT
Hairpin Sequence:
5'-CCGG-CTAGACACTGAGGGCCGATAT-CTCGAG-ATATCGGCCCTCAGTGTCTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAGACACTGAGGGCCGATATCTCGAGATATCGGCCCTCAGTGTCTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAGACACTGAGGGCCGATATCTCGAGATATCGGCCCTCAGTGTCTAG-3'

Other clones with same target sequence:

(none)