Construct: shRNA TRCN0000235219
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_975064.1-1882s21c1
- DNA Barcode:
- ACAGGAGAGAAACCCTATAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC66376 (66376)
- Gene Description:
- uncharacterized LOC66376
- Transcript:
- RefSeq XM_975064.1 (NON-CURRENT)
- Match location:
- Position 1882 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000235219)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- ACAGGAGAGAAACCCTATAAA
- Hairpin Sequence:
-
5'-CCGG-ACAGGAGAGAAACCCTATAAA-CTCGAG-TTTATAGGGTTTCTCTCCTGT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGACAGGAGAGAAACCCTATAAACTCGAGTTTATAGGGTTTCTCTCCTGTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAACAGGAGAGAAACCCTATAAACTCGAGTTTATAGGGTTTCTCTCCTGT-3'
Other clones with same target sequence:
-
TRCN0000235327
