Construct: shRNA TRCN0000235264

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_974517.1-269s21c1
DNA Barcode:
CCTCACTGCCATAGGGTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
EG665088 (665088)
Gene Description:
predicted gene, EG665088
Transcript:
RefSeq XM_974517.1 (NON-CURRENT)
Match location:
Position 269 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235264)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCACTGCCATAGGGTATAA
Hairpin Sequence:
5'-CCGG-CCTCACTGCCATAGGGTATAA-CTCGAG-TTATACCCTATGGCAGTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCACTGCCATAGGGTATAACTCGAGTTATACCCTATGGCAGTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCACTGCCATAGGGTATAACTCGAGTTATACCCTATGGCAGTGAGG-3'

Other clones with same target sequence:

(none)