Construct: shRNA TRCN0000235334

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_975229.1-1533s21c1
DNA Barcode:
CACTACTCCAAGGGAGTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Thap6 (381650)
Gene Description:
THAP domain containing 6
Transcript:
RefSeq XM_975229.1 (NON-CURRENT)
Match location:
Position 1533 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235334)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTACTCCAAGGGAGTTAAA
Hairpin Sequence:
5'-CCGG-CACTACTCCAAGGGAGTTAAA-CTCGAG-TTTAACTCCCTTGGAGTAGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTACTCCAAGGGAGTTAAACTCGAGTTTAACTCCCTTGGAGTAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTACTCCAAGGGAGTTAAACTCGAGTTTAACTCCCTTGGAGTAGTG-3'

Other clones with same target sequence:

(none)