Construct: shRNA TRCN0000235396

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016631.3-2518s21c1
DNA Barcode:
AGTTAAGCTGTTAGGCAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAXBP1 (94104)
Gene Description:
PAX3 and PAX7 binding protein 1
Transcript:
RefSeq NM_016631.3 (NON-CURRENT)
Match location:
Position 2518 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235396)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTTAAGCTGTTAGGCAATTT
Hairpin Sequence:
5'-CCGG-AGTTAAGCTGTTAGGCAATTT-CTCGAG-AAATTGCCTAACAGCTTAACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTAAGCTGTTAGGCAATTTCTCGAGAAATTGCCTAACAGCTTAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTAAGCTGTTAGGCAATTTCTCGAGAAATTGCCTAACAGCTTAACT-3'

Other clones with same target sequence:

(none)