Construct: shRNA TRCN0000235422

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018315.4-451s21c1
DNA Barcode:
ATGGGTTTCTACGGCACATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXW7 (55294)
Gene Description:
F-box and WD repeat domain containing 7
Transcript:
RefSeq NM_018315.4 (NON-CURRENT)
Match location:
Position 451 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235422)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGGTTTCTACGGCACATTA
Hairpin Sequence:
5'-CCGG-ATGGGTTTCTACGGCACATTA-CTCGAG-TAATGTGCCGTAGAAACCCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGGTTTCTACGGCACATTACTCGAGTAATGTGCCGTAGAAACCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGGTTTCTACGGCACATTACTCGAGTAATGTGCCGTAGAAACCCAT-3'

Other clones with same target sequence:

  1. TRCN0000365964