Construct: shRNA TRCN0000235423

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018315.4-1515s21c1
DNA Barcode:
TGATGGCAGGAGGGTTGTTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXW7 (55294)
Gene Description:
F-box and WD repeat domain containing 7
Transcript:
RefSeq NM_018315.4 (NON-CURRENT)
Match location:
Position 1515 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235423)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATGGCAGGAGGGTTGTTAG
Hairpin Sequence:
5'-CCGG-TGATGGCAGGAGGGTTGTTAG-CTCGAG-CTAACAACCCTCCTGCCATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATGGCAGGAGGGTTGTTAGCTCGAGCTAACAACCCTCCTGCCATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATGGCAGGAGGGTTGTTAGCTCGAGCTAACAACCCTCCTGCCATCA-3'

Other clones with same target sequence:

(none)