Construct: shRNA TRCN0000235424

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018315.4-644s21c1
DNA Barcode:
GGCAACAACGACGCCGAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXW7 (55294)
Gene Description:
F-box and WD repeat domain containing 7
Transcript:
RefSeq NM_018315.4 (NON-CURRENT)
Match location:
Position 644 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235424)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCAACAACGACGCCGAATTA
Hairpin Sequence:
5'-CCGG-GGCAACAACGACGCCGAATTA-CTCGAG-TAATTCGGCGTCGTTGTTGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCAACAACGACGCCGAATTACTCGAGTAATTCGGCGTCGTTGTTGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCAACAACGACGCCGAATTACTCGAGTAATTCGGCGTCGTTGTTGCC-3'

Other clones with same target sequence:

(none)