Construct: shRNA TRCN0000235461

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006226.2-3636s21c1
DNA Barcode:
GATCTTAGCATAGCTTATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLCL1 (5334)
Gene Description:
phospholipase C like 1 (inactive)
Transcript:
RefSeq NM_006226.2 (NON-CURRENT)
Match location:
Position 3636 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235461)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCTTAGCATAGCTTATTAT
Hairpin Sequence:
5'-CCGG-GATCTTAGCATAGCTTATTAT-CTCGAG-ATAATAAGCTATGCTAAGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCTTAGCATAGCTTATTATCTCGAGATAATAAGCTATGCTAAGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCTTAGCATAGCTTATTATCTCGAGATAATAAGCTATGCTAAGATC-3'

Other clones with same target sequence:

(none)