Construct: shRNA TRCN0000235511

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018091.5-653s21c1
DNA Barcode:
GTGATGGGTGGAACGTTTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ELP3 (55140)
Gene Description:
elongator acetyltransferase complex subunit 3
Transcript:
RefSeq NM_018091.5 (NON-CURRENT)
Match location:
Position 653 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235511)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGATGGGTGGAACGTTTATG
Hairpin Sequence:
5'-CCGG-GTGATGGGTGGAACGTTTATG-CTCGAG-CATAAACGTTCCACCCATCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGATGGGTGGAACGTTTATGCTCGAGCATAAACGTTCCACCCATCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGATGGGTGGAACGTTTATGCTCGAGCATAAACGTTCCACCCATCAC-3'

Other clones with same target sequence:

(none)