Construct: shRNA TRCN0000235513
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_002716.4-804s21c1
- DNA Barcode:
- TGTCAGTATTGCCCAGTTATT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PPP2R1B (5519)
- Gene Description:
- protein phosphatase 2 scaffold subunit Abeta
- Transcript:
- RefSeq NM_002716.4 (NON-CURRENT)
- Match location:
- Position 804 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000235513)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TGTCAGTATTGCCCAGTTATT
- Hairpin Sequence:
-
5'-CCGG-TGTCAGTATTGCCCAGTTATT-CTCGAG-AATAACTGGGCAATACTGACA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTGTCAGTATTGCCCAGTTATTCTCGAGAATAACTGGGCAATACTGACATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATGTCAGTATTGCCCAGTTATTCTCGAGAATAACTGGGCAATACTGACA-3'
Other clones with same target sequence:
(none)
