Construct: shRNA TRCN0000235516

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002716.4-300s21c1
DNA Barcode:
TGATGAAGATGAGGTACTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP2R1B (5519)
Gene Description:
protein phosphatase 2 scaffold subunit Abeta
Transcript:
RefSeq NM_002716.4 (NON-CURRENT)
Match location:
Position 300 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235516)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATGAAGATGAGGTACTATT
Hairpin Sequence:
5'-CCGG-TGATGAAGATGAGGTACTATT-CTCGAG-AATAGTACCTCATCTTCATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATGAAGATGAGGTACTATTCTCGAGAATAGTACCTCATCTTCATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATGAAGATGAGGTACTATTCTCGAGAATAGTACCTCATCTTCATCA-3'

Other clones with same target sequence:

(none)