Construct: shRNA TRCN0000235521

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000264.3-588s21c1
DNA Barcode:
GGACGAGTAAGTCGTGAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTCH1 (5727)
Gene Description:
patched 1
Transcript:
RefSeq NM_000264.3 (NON-CURRENT)
Match location:
Position 588 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235521)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGACGAGTAAGTCGTGAATTA
Hairpin Sequence:
5'-CCGG-GGACGAGTAAGTCGTGAATTA-CTCGAG-TAATTCACGACTTACTCGTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGACGAGTAAGTCGTGAATTACTCGAGTAATTCACGACTTACTCGTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGACGAGTAAGTCGTGAATTACTCGAGTAATTCACGACTTACTCGTCC-3'

Other clones with same target sequence:

(none)