Construct: shRNA TRCN0000235528

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001433.3-363s21c1
DNA Barcode:
ACGCTTGGAAGCAAGAATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ERN1 (2081)
Gene Description:
endoplasmic reticulum to nucleus signaling 1
Transcript:
RefSeq NM_001433.3 (NON-CURRENT)
Match location:
Position 363 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235528)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGCTTGGAAGCAAGAATAAT
Hairpin Sequence:
5'-CCGG-ACGCTTGGAAGCAAGAATAAT-CTCGAG-ATTATTCTTGCTTCCAAGCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGCTTGGAAGCAAGAATAATCTCGAGATTATTCTTGCTTCCAAGCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGCTTGGAAGCAAGAATAATCTCGAGATTATTCTTGCTTCCAAGCGT-3'

Other clones with same target sequence:

(none)