Construct: shRNA TRCN0000235555

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013685.2-2253s21c1
DNA Barcode:
GAACGGAGGATGGCCAATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tcf4 (21413)
Gene Description:
transcription factor 4
Transcript:
RefSeq NM_013685.2 (CURRENT)
Match location:
Position 2253 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235555)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACGGAGGATGGCCAATAAT
Hairpin Sequence:
5'-CCGG-GAACGGAGGATGGCCAATAAT-CTCGAG-ATTATTGGCCATCCTCCGTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACGGAGGATGGCCAATAATCTCGAGATTATTGGCCATCCTCCGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACGGAGGATGGCCAATAATCTCGAGATTATTGGCCATCCTCCGTTC-3'

Other clones with same target sequence:

(none)