Construct: shRNA TRCN0000235591

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018988.2-712s21c1
DNA Barcode:
TCATCATCCCGCTGCTGAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GFOD1 (54438)
Gene Description:
glucose-fructose oxidoreductase domain containing 1
Transcript:
RefSeq NM_018988.2 (NON-CURRENT)
Match location:
Position 712 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235591)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATCATCCCGCTGCTGAAAG
Hairpin Sequence:
5'-CCGG-TCATCATCCCGCTGCTGAAAG-CTCGAG-CTTTCAGCAGCGGGATGATGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATCATCCCGCTGCTGAAAGCTCGAGCTTTCAGCAGCGGGATGATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATCATCCCGCTGCTGAAAGCTCGAGCTTTCAGCAGCGGGATGATGA-3'

Other clones with same target sequence:

(none)