Construct: shRNA TRCN0000235748

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012074.3-1052s21c1
DNA Barcode:
GGTTCGCCCACAGCAGATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DPF3 (8110)
Gene Description:
double PHD fingers 3
Transcript:
RefSeq NM_012074.3 (NON-CURRENT)
Match location:
Position 1052 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235748)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTTCGCCCACAGCAGATAAA
Hairpin Sequence:
5'-CCGG-GGTTCGCCCACAGCAGATAAA-CTCGAG-TTTATCTGCTGTGGGCGAACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTTCGCCCACAGCAGATAAACTCGAGTTTATCTGCTGTGGGCGAACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTTCGCCCACAGCAGATAAACTCGAGTTTATCTGCTGTGGGCGAACC-3'

Other clones with same target sequence:

(none)