Construct: shRNA TRCN0000235775

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010118.3-519s21c1
DNA Barcode:
GAGATGGCATGATCAACATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Egr2 (13654)
Gene Description:
early growth response 2
Transcript:
RefSeq NM_010118.3 (CURRENT)
Match location:
Position 519 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235775)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATGGCATGATCAACATTG
Hairpin Sequence:
5'-CCGG-GAGATGGCATGATCAACATTG-CTCGAG-CAATGTTGATCATGCCATCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATGGCATGATCAACATTGCTCGAGCAATGTTGATCATGCCATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATGGCATGATCAACATTGCTCGAGCAATGTTGATCATGCCATCTC-3'

Other clones with same target sequence:

(none)