Construct: shRNA TRCN0000235847

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011544.3-1044s21c1
DNA Barcode:
ATGTCTCAGTCCAGTAGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tcf12 (21406)
Gene Description:
transcription factor 12
Transcript:
RefSeq NM_011544.3 (CURRENT)
Match location:
Position 1044 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235847)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTCTCAGTCCAGTAGTTAT
Hairpin Sequence:
5'-CCGG-ATGTCTCAGTCCAGTAGTTAT-CTCGAG-ATAACTACTGGACTGAGACAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTCTCAGTCCAGTAGTTATCTCGAGATAACTACTGGACTGAGACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTCTCAGTCCAGTAGTTATCTCGAGATAACTACTGGACTGAGACAT-3'

Other clones with same target sequence:

(none)