Construct: shRNA TRCN0000235918

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173082.2-1426s21c1
DNA Barcode:
GGTCTGAAACTCTACTATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SHPRH (257218)
Gene Description:
SNF2 histone linker PHD RING helicase
Transcript:
RefSeq NM_173082.2 (NON-CURRENT)
Match location:
Position 1426 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235918)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCTGAAACTCTACTATAAT
Hairpin Sequence:
5'-CCGG-GGTCTGAAACTCTACTATAAT-CTCGAG-ATTATAGTAGAGTTTCAGACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCTGAAACTCTACTATAATCTCGAGATTATAGTAGAGTTTCAGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCTGAAACTCTACTATAATCTCGAGATTATAGTAGAGTTTCAGACC-3'

Other clones with same target sequence:

(none)