Construct: shRNA TRCN0000235955

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081376.1-895s21c1
DNA Barcode:
CAGCGCCAACAACCCGTTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chd5 (269610)
Gene Description:
chromodomain helicase DNA binding protein 5
Transcript:
RefSeq NM_001081376.1 (CURRENT)
Match location:
Position 895 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000235955)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCGCCAACAACCCGTTTAA
Hairpin Sequence:
5'-CCGG-CAGCGCCAACAACCCGTTTAA-CTCGAG-TTAAACGGGTTGTTGGCGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCGCCAACAACCCGTTTAACTCGAGTTAAACGGGTTGTTGGCGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCGCCAACAACCCGTTTAACTCGAGTTAAACGGGTTGTTGGCGCTG-3'

Other clones with same target sequence:

(none)